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Related Experiment Video

Updated: Jun 13, 2026

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
08:28

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Published on: June 30, 2017

Cord lining progenitor cells: potential in vitro adipogenesis model.

H H Cheong1, J Masilamani, T T Phan

  • 1Department of Pharmacy, National University of Singapore, Singapore, Singapore.

International Journal of Obesity (2005)
|May 19, 2010
PubMed
Summary

Umbilical cord lining cells differentiate into adipocyte-like cells (ALCs). Glucose and insulin levels influence ALC secretions, suggesting potential for studying obesity and metabolic disorders.

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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues
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Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues

Published on: August 25, 2020

Related Experiment Videos

Last Updated: Jun 13, 2026

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
08:28

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Published on: June 30, 2017

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues
06:17

Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues

Published on: August 25, 2020

Area of Science:

  • Cell Biology
  • Metabolic Research

Background:

  • Adipogenesis is crucial for energy homeostasis.
  • Understanding progenitor cell differentiation is key to metabolic research.

Purpose of the Study:

  • Investigate glucose and insulin effects on umbilical cord lining progenitor cell differentiation into adipocyte-like cells (ALCs).

Main Methods:

  • Isolated cord lining mesenchymal cells (CLMCs) and induced differentiation.
  • Confirmed lipid droplet formation using Oil Red O staining.
  • Analyzed gene expression (adipsin, PPARγ) and protein secretion (leptin, adiponectin).

Main Results:

  • CLMCs differentiated into ALCs with upregulated adipsin and PPARγ.
  • ALCs in low-glucose medium secreted leptin and adiponectin; high-glucose medium led to leptin secretion only.
  • Insulin affected leptin secretion but not adiponectin.

Conclusions:

  • CLMCs can differentiate into ALCs under varying culture conditions.
  • These ALCs serve as a viable in vitro model for adipogenesis and obesity studies.
  • Potential application in drug discovery for metabolic disorders.