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Updated: Jun 12, 2026
07:01
Impression Cytology of the Lid Wiper Area
Published on: August 9, 2016
Control specimens for immunocytochemistry in liquid-based cytology
T Hansen1, H Pedersen, V Brauner
1Department of Pathology, Sygehus Sønderjylland, Sønderborg, Denmark. tovelhansen@gmail.com
Summary
Developing control specimens for immunocytochemistry (ICC) using liquid-based cytology (LBC) is now feasible. Fresh tissue and body fluid samples can be processed into slides and stored for at least 40 days without losing antigenicity.
Area of Science:
- Cytopathology
- Immunohistochemistry
- Molecular Diagnostics
Background:
- Immunohistochemistry (IHC) controls are standard, but suitable immunocytochemistry (ICC) controls are challenging to establish.
- Existing literature lacks comprehensive methods for preparing ICC control specimens.
Purpose of the Study:
- To develop and validate a method for selecting, collecting, processing, and storing control specimens for ICC using liquid-based cytology (LBC).
Main Methods:
- Utilized 21 antibodies targeting cellular components from tonsils, serous fluids, and bronchial washings.
- Collected tonsil specimens via brushing into SurePath™ vials; fixed serous fluids and bronchial washings in CytoRich Red™.
- Prepared unstained SurePath™ slides, stored them at 5°C for up to 40 days, and performed ICC.
Main Results:
- Unstained SurePath™ slides demonstrated positive reactivity with relevant antibodies.
- Stored slides maintained staining intensity for up to 40 days, indicating preserved antigenicity.
Conclusions:
- Body fluid and cell-suspension specimens from fresh tissues are effective as ICC controls, individually or as mixtures.
- Dry, unstained slides can be stored refrigerated for at least 40 days without compromising antigenicity for ICC.
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