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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
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Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
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IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
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Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

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Chemical identity testing by remote-based dispersive Raman spectroscopy.

David E Bugay1, Robert C Brush

  • 1PharmAnalysis, Inc., 2717 N CR 475 West, West Lafayette, Indiana 47906, USA. david.bugay@comcast.net

Applied Spectroscopy
|May 21, 2010
PubMed
Summary

Hand-held Raman spectrometers enable portable pharmaceutical analysis. A new algorithm enhances the precision and reliability of chemical identification methods, ensuring accurate drug product verification.

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A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
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Last Updated: Jun 12, 2026

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Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Sciences
  • Spectroscopy

Background:

  • Current Good Manufacturing Practices (cGMP) compliant hand-held Raman spectrometers offer new possibilities for pharmaceutical analysis.
  • Bringing laboratory capabilities to the sample site streamlines analytical workflows.

Purpose of the Study:

  • To explore the application of hand-held Raman spectrometers for qualitative chemical identification of pharmaceutical products.
  • To develop and validate robust methods for drug identification using portable Raman technology.

Main Methods:

  • Development of qualitative chemical identification methods on two different hand-held Raman spectrometers.
  • Transfer of developed methods to a third instrument for validation.
  • Application of a novel decision algorithm for spectral correlation assessment, emphasizing precision and reliability.

Main Results:

  • Successful development and transfer of chemical identification assays using hand-held Raman spectrometers.
  • Demonstration of the reliability of portable Raman spectroscopy for pharmaceutical analysis.
  • The novel algorithm effectively reduces human bias in spectral comparison.

Conclusions:

  • Hand-held Raman spectrometers are reliable tools for developing, validating, and transferring chemical identification assays in the pharmaceutical industry.
  • The presented decision algorithm improves the objectivity and accuracy of spectral identification.
  • Portable Raman spectroscopy facilitates efficient and reliable on-site analysis of drug products.