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Monolithic PDMS passband filters for fluorescence detection.

Andreu Llobera1, Stefanie Demming, Haakan N Joensson

  • 1Institut für Mikrotechnik, Technische Universität Braunschweig, Alte Salzdahlumer Str. 203, 38124 Braunschweig, Germany.

Lab on a Chip
|May 21, 2010
PubMed
Summary

We developed low-cost, integrated optical filters using ink-dyed poly(dimethylsiloxane) (PDMS) for disposable lab-on-a-chip devices. These robust filters enable portable optical sensing without temporal aging, enhancing biological and chemical analyses.

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Area of Science:

  • Microfluidics
  • Optical Engineering
  • Materials Science

Background:

  • Disposable lab-on-a-chip (LOC) devices require integrated optical functionalities for portability.
  • Current LOC systems often rely on external components, limiting true portability and increasing costs.

Purpose of the Study:

  • To develop and characterize monolithically integrated, ink-dyed poly(dimethylsiloxane) (PDMS) optical filters for LOC devices.
  • To enable portable, low-cost optical sensing by migrating auxiliary functions onto the disposable chip.

Main Methods:

  • Filters fabricated by directly mixing commercial ink into PDMS oligomer, patterned using soft lithography and capillary forces.
  • Characterization of filters with varying thicknesses and ink concentrations (red, green, blue).

Main Results:

  • Optimal performance achieved at 250 µm thickness and 0.1 ink:PDMS ratio.
  • Achieved transmittance of -15.1 to -12.3 dB (stopband) and -4.0 to -2.5 dB (passband).
  • Demonstrated robustness against temporal aging and successful integration in fluorescence systems.

Conclusions:

  • Ink-dyed PDMS filters offer a robust, low-cost solution for integrated optical functionalities in disposable microdevices.
  • These filters enable a wider range of fluorescence and absorbance-based analyses on portable LOC platforms.