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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

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Rapid determination of transgene copy number in tobacco by competitive PCR using a pair of SSR primers

K Y Xu1, S H Wang, L Xi

  • 1College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, China.

Genetics and Molecular Research : GMR
|May 21, 2010
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...