[Effect of 1-methylnicotinamide on prooxidant-antioxidant balance parameters in rats during hepatic...

We have studied the effect of 1-methylnicotinamide (MNA) on prooxidant - antioxidant balance parameters by measuring the concentrations of lipid peroxidation products (conjugated dienes (CD) and Schiff bases (SB)) and antioxidant system factors (alpha-tocoferol (alpha-T), retinol (Ret) in the blood and liver homogenates and by evaluating the activity of catalase (Cat) and alanine and aspartate aminotransferases in the blood plasma in the course of hepatic ischemia-reperfusion in a group of 16 adult male Wistar rats (weighing 360-440 g). The anmals were divided into two groups: (1) hepatic ischemia (30 min, m. Pringle) and reperfusion (120 min) (HIR, n = 8); (2) HIR with MNA (100 mg/kg. i.p. 10 min before HIR, n = 8). In the first group, the plasma level of CD raised to 264.4% (p < 0.05), SB to 633.3% (p < 0.05), the concentration of alpha-T decreased by 19% (p < 0.05), that of Ret by 35.8 % (p < 0.05), and Cat by 45.8 % (p < 0.05) at the end of reperfusion as compared to control values before ischemia. In the second group, at the end of reperfusion the level of CD increased only to 51.4% (p < 0.05), SB to 130.3% (p < 0.05), Cat to 65% (p < 0.05), and the concentration of alpha-T in the plasma decreased only to 11% (p < 0.05), and Ret to 20.7% (p < 0.05). It is concluded that MNA significantly improves the prooxidant - antioxidant balance parameters during hepatic ischemia-reperfusion in rats.