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Updated: Jun 12, 2026
Murine Bilateral Renal Lymphadenectomy
Published on: December 30, 2025
Mesenteric lymph duct ligation against renal injury in rats after hemorrhagic shock
Chun-Yu Niu1, Zi-Gang Zhao, Yun-Long Ye
1Institute of Microcirculation, Hebei North University, Hebei, Zhangjiakou 075029, China. ncylxf@126.com
Background:
The kidney is a common target in multiple organ dysfunction syndrome (MODS). The aim of this study is to determine the role of intestinal lymphatic pathway on renal injury in hemorrhagic shock rats.
Methods:
Wistar rats were divided into sham, shock, and ligation groups. The hemorrhagic shock model was induced in the shock and ligation groups. After resuscitation, the mesenteric lymph ducts were ligated in the ligation group. Blood from the carotid artery was taken to determine renal functional indices. The kidneys were used to observe histomorphological changes at 6 h after resuscitation. In addition, kidney homogenate was used to determine malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6), and myeloperoxidase (MPO) levels at 90 min after shock and 0, 1, 3, 6, 12, and 24 h after resuscitation. And the survival rate of 24 h was recorded.
Results:
The survival rate in shock group was obviously lower than sham and ligation groups. The urea and creatinine contents in the serum of shock and ligation groups were significantly higher than the sham group; the indices in the ligation group were significantly lower than the shock group. Histological studies showed various degrees of renal injury in the shock and ligation groups with a lesser severity in the ligation group. MDA, TNFalpha, IL-6, and MPO in renal homogenate of the shock group were raised, and the activity of SOD was lower in comparison to the sham group. Further, MDA, TNFalpha, IL-6, and MPO in renal homogenate of the ligation group at 6, 12, and 24 h were lower, and the SOD activity was higher than that of the shock group at the same time points.
Conclusion:
The mesenteric lymph duct ligation could be used to attenuate renal injury in shock rats. Its mechanism might be related to reducing the polymorph nuclear (PMN) and decreasing inflammatory mediator and free radical.
Insights
Ligating the intestinal lymphatic pathway protected kidneys from hemorrhagic shock in rats. This intervention reduced inflammatory markers and free radicals, improving survival and renal function.
Area of Science:
- Physiology
- Pathology
- Surgical Research
Background:
- The kidney is a frequent target organ in multiple organ dysfunction syndrome (MODS).
- Hemorrhagic shock can lead to significant renal injury.
- The role of the intestinal lymphatic pathway in this process requires further elucidation.
Purpose of the Study:
- To investigate the protective effect of the intestinal lymphatic pathway on renal injury in a rat model of hemorrhagic shock.
- To determine if ligating the mesenteric lymph ducts can attenuate kidney damage following hemorrhagic shock and resuscitation.
Main Methods:
- Wistar rats underwent sham operation, hemorrhagic shock induction, or hemorrhagic shock followed by mesenteric lymph duct ligation.
- Renal function was assessed by serum urea and creatinine levels.
- Kidney tissues were analyzed for histomorphological changes and levels of malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and myeloperoxidase (MPO).
- Survival rates were recorded at 24 hours.
Main Results:
- Mesenteric lymph duct ligation significantly improved 24-hour survival rates compared to the shock-only group.
- Renal function indices (urea, creatinine) were significantly better in the ligation group than the shock group.
- Histological examination revealed less severe renal injury in ligated rats.
- Markers of oxidative stress and inflammation (MDA, TNF-alpha, IL-6, MPO) were reduced, while antioxidant activity (SOD) was preserved in the ligation group post-resuscitation.
Conclusions:
- Ligation of the mesenteric lymph ducts effectively attenuates renal injury in rats subjected to hemorrhagic shock.
- This protective effect may be attributed to the reduction of polymorphonuclear cell infiltration, inflammatory mediators, and free radicals via the intestinal lymphatic pathway.
