Clinical implications of the apolipoprotein E polymorphism and genetic variants: current methods for apo E
1SERLIA, Institut Pasteur, Lille.
Annales De Biologie Clinique
|January 1, 1991
Summary
Apolipoprotein E (apo E) genotype influences lipid levels, with apo E2 linked to specific conditions. Understanding apo E phenotype may help predict atherosclerosis risk.
Area of Science:
- Genetics
- Biochemistry
- Cardiovascular Disease
Background:
- Apolipoprotein E (apo E) genetic polymorphism significantly impacts human plasma lipid and apoprotein levels.
- The apo E2 isoform is strongly associated with primary dysbetalipoproteinemia and hyperlipoproteinemia type III.
- The roles of apo E2 in lowering cholesterol and apo E4 in raising cholesterol concerning coronary heart disease risk remain debated.
Purpose of the Study:
- To refine methods for analyzing apolipoprotein E (apo E) polymorphism and phenotyping.
- To improve the assessment of individual atherosclerosis risk based on apo E phenotype.
- To develop novel approaches for apo E isoform analysis.
Main Methods:
- Modification of three existing procedures for apo E isoform analysis.
- Techniques for visualizing apo E patterns in various solution conditions (dilute, high salt).
- Large-scale apo E phenotyping from whole plasma, addressing potential thrombin fragment interference.
- High-resolution analysis using immobilized pH gradient gels to detect new apo E variants.
Main Results:
- Developed modified methods for accurate apo E phenotyping.
- Enhanced visualization techniques for apo E patterns.
- Established a reliable large-scale phenotyping method from plasma.
- Initiated the search for novel apo E variants using advanced gel electrophoresis.
Conclusions:
- Apolipoprotein E (apo E) phenotyping offers valuable insights into lipid metabolism and cardiovascular risk.
- Modified analytical methods improve the accuracy and scope of apo E isoform analysis.
- Further research into apo E variants may enhance atherosclerosis risk prediction.
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