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Published on: September 11, 2015
Bone tissue engineering using porous carbonate apatite and bone marrow cells
Tadakatsu Kasai1, Kimihiko Sato, Yoshinori Kanematsu
1Division of Oral Pathogenesis and Disease Control, Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Mizuho City, Gifu, Japan. kasai@dent.asahi-u.ac.jp
Porous carbonate apatite blocks seeded with bone marrow cells successfully induced new bone formation in rats. This hybrid biomaterial shows potential for heterotopic bone regeneration in vivo.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Porous carbonate apatite (CA) scaffolds are investigated for bone regeneration.
- Stem cells within bone marrow possess osteogenic potential.
Purpose of the Study:
- To evaluate the osteoinductive capacity of a hybrid biomaterial composed of porous CA blocks and rat bone marrow cells.
- To assess heterotopic bone formation in vivo using this hybrid construct.
Main Methods:
- Porous CA blocks (300-500 microm particles) were fabricated via sintering at 750°C.
- Rat bone marrow cells were cultured on CA blocks for 14 days to promote osteoblast differentiation.
- Hybrid constructs were implanted into the subcutaneous tissue of syngeneic rats.
Main Results:
- Microfocus X-ray computed tomography revealed new bone formation at the extraosseous implantation site.
- Bone formation was observed at 4 and 8 weeks post-implantation.
Conclusions:
- The hybrid of porous carbonate apatite and bone marrow cells effectively induces heterotopic bone formation.
- This biomaterial strategy holds promise for bone regenerative applications.
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