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Updated: Jun 12, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Angstrom resolution optical profilometry for microscopic objects
Abstract:
An instrument capable of recording the amplitude and phase of reflected light with a phase resolution of better than lambda/3000 and the lateral resolution of a confocal scanning microscope was built. The instrument is based on a commercial microscope body and uses the regular interference contrast optics. The modifications consisted of adding a coherent (heterodyne) detector and a confocal laser scanning system. Two-dimensional surface images of amplitude, slope, and profile were taken with a step height resolution of typically 0.5-2 A. The instrument is described, and its characteristics for surface profilometry are discussed.
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