Fragmentation-fluorescence spectrometric determination of nonfluorescent compounds

Area of Science:

• Analytical Chemistry
• Spectroscopy
• Photochemistry

Background:

• Nonfluorescent organic and organometallic compounds lack intrinsic fluorescence, limiting their detection by conventional fluorometric methods.
• Developing sensitive and selective detection techniques for these compounds is crucial in various scientific fields.

Purpose of the Study:

• To describe a novel method for the fluorometric detection and quantification of nonfluorescent compounds using molecular fragmentation.
• To investigate the use of electron impact and laser photolysis for fragment generation.

Main Methods:

• Molecular fragmentation induced by 100-eV electrons or 193-nm photons.
• Detection and quantification based on the emissive properties of generated molecular fragments (e.g., OH, CN, CH).
• Comparison of laser photolysis and electron impact techniques for fragment generation.

Main Results:

• Demonstration of fluorometric detection for previously nonfluorescent analytes.
• Characterization of detection limits, linearity, reproducibility, and applicability range.
• Comparative analysis of electron impact and laser photolysis for generating fluorescent fragments.

Conclusions:

• Molecular fragmentation is a viable technique for the sensitive fluorometric detection of nonfluorescent organic and organometallic compounds.
• Both electron impact and laser photolysis are effective methods for inducing fragmentation and enabling detection.
• The described method offers a new analytical approach for a wide range of nonfluorescent analytes.