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Updated: Jun 12, 2026

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Published on: November 27, 2019

Commonly adopted caloric restriction protocols often involve malnutrition.

Fernanda M Cerqueira¹, Alicia J Kowaltowski

¹Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil. fmeneses@iq.usp.br

Ageing Research Reviews

|May 25, 2010

Summary

Caloric restriction diets often lead to malnutrition in lab animals due to inadequate micronutrient levels. Researchers propose redefining "caloric restriction" to ensure both calorie and micronutrient adequacy for accurate longevity studies.

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Last Updated: Jun 12, 2026

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

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Area of Science:

Gerontology
Nutritional Science
Laboratory Animal Science

Background:

Caloric restriction (CR) is a widely studied intervention for enhancing laboratory animal lifespan.
Current CR protocols are often poorly defined and may not meet essential nutritional requirements.

Purpose of the Study:

To evaluate if common CR protocols provide adequate micronutrients for laboratory rodents.
To clarify the definition and application of CR in longevity research.

Main Methods:

Literature review of CR protocols over four years.
Analysis of micronutrient content in commonly used CR diets (e.g., 40% AIN-93 without supplementation).
Examination of feeding schedules like every-other-day feeding.

Main Results:

Most CR protocols reviewed do not meet National Research Council micronutrient standards for rats and mice.
A 40% restriction of the AIN-93 diet without supplementation causes malnutrition in rodents.
Every-other-day feeding does not necessarily reduce total calorie intake.

Conclusions:

The term "caloric restriction" should specifically denote diets reducing calories without sacrificing micronutrient availability.
Detailed reporting of CR protocols is crucial for reproducible and comparable longevity research.
Ensuring nutritional adequacy is vital for accurate interpretation of CR's effects on lifespan.