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Succinic acid production with metabolically engineered E. coli recovered from two-stage fermentation
Jiang-Feng Ma1, Min Jiang, Ke-Quan Chen
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, Nanjing, 210009, China.
Biotechnology Letters
|May 25, 2010
Summary
Recovered Escherichia coli cells efficiently produced succinic acid anaerobically with only glucose and MgCO(3) supplementation. These reusable cells achieved high yields and productivity comparable to initial fermentation, suggesting potential for cost-effective bioproduction.
Area of Science:
- Biotechnology
- Metabolic Engineering
- Industrial Microbiology
Background:
- Efficient production of succinic acid is crucial for its industrial applications.
- Recycling microbial cells can reduce bioprocess costs and environmental impact.
- Escherichia coli is a well-established platform for producing organic acids.
Purpose of the Study:
- To investigate the potential of recovered Escherichia coli AFP111 cells for additional succinic acid production.
- To determine the influence of initial glucose concentration and cell density on succinic acid yield and productivity.
- To evaluate the feasibility of reusing recovered cells for sustainable succinic acid biosynthesis.
Main Methods:
- Cultivation of Escherichia coli AFP111 in a two-stage fermentation process.
- Recovery of cells from spent fermentation broth.
- Anaerobic cultivation of recovered cells with glucose and MgCO(3) supplementation.
- Optimization of initial glucose concentration and cell density.
- Analysis of succinic acid mass yield and productivity.
Main Results:
- Recovered cells produced succinic acid anaerobically without additional nutrient supplementation beyond glucose and MgCO(3).
- Initial glucose concentration and cell density significantly impacted succinic acid mass yield and productivity.
- After three rounds of recycling, an average succinic acid mass yield of 0.85 g/g and productivity of 1.81 g/l h were achieved.
- While final succinic acid concentration was lower than in the initial two-stage fermentation, the yield and productivity were comparable.
Conclusions:
- Recovered Escherichia coli AFP111 cells can be effectively reused for succinic acid production.
- The process demonstrates a sustainable approach to succinic acid biosynthesis with reduced costs.
- Further optimization could enhance the overall efficiency of cell recycling for industrial bioproduction.
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