Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ctxR: Utilities for interacting with the CTX APIs.

NAM journal·2026
Same author

Cost-effectiveness of emergency department opt-out testing for HIV in England: a modelling study.

The lancet. HIV·2026
Same author

Geospatial correlation of industrial plastics emissions with bladder and kidney cancer burden in Ohio.

Urologic oncology·2026
Same author

Bladder Cancer, Version 1.2026, NCCN Clinical Practice Guidelines In Oncology.

Journal of the National Comprehensive Cancer Network : JNCCN·2026
Same author

Patterns of local therapy following enfortumab vedotin in advanced urothelial carcinoma.

Urologic oncology·2026
Same author

Immune checkpoint inhibitors in urothelial carcinoma: a practical framework for patient selection, toxicity management, response assessment, and treatment sequencing.

Expert review of anticancer therapy·2026
Same journal

Synergistic Impact of Turkey Red Oil and Sodium Oleate on the Separation of Dolomite from Apatite.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Metal Substrate-Dependent Tribological Performance of Environmentally Acceptable Ester-PAO Lubricant Blends.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

From Waste to Water Remediation: Fly Ash-Derived Hectorite for Dye and Heavy Metal Removal.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Mechanism of the Cholesterol-dependent Anchoring and Conformation of LPP-scFv on the PEGylated Liposome Surface.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Visualizing Cooperative Adsorption of an Enzyme Mixture at an Air-Liquid Interface.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Unraveling Nanoplastics-Enzyme Interactions: Physicochemical, Structural, Functional, and Cell Biological Characterization of α-Amylase-Nanoplastics Complexes.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Kinetic parameters from detection probability in single molecule force spectroscopy.

Chad Ray1, Senli Guo, Jason Brown

  • 1Department of Chemistry, Duke University, Durham, North Carolina 27708, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|May 26, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a new method using detection probability in atomic force microscopy force spectroscopy to determine molecular bond dissociation kinetics. The optimal probe velocity for detecting bond rupture is independent of dissociation rate, offering a complementary approach to existing techniques.

More Related Videos

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Related Experiment Videos

Last Updated: Jun 12, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Area of Science:

  • Biophysics
  • Materials Science
  • Physical Chemistry

Background:

  • Atomic force microscopy (AFM) force spectroscopy is crucial for studying molecular interactions.
  • Extracting kinetic parameters of bond dissociation typically relies on standard methods.
  • Limitations exist in current methods for analyzing rupture events.

Purpose of the Study:

  • To develop and validate a novel model for extracting kinetic parameters of dissociation using detection probability in AFM force spectroscopy.
  • To establish a method complementary to existing approaches, particularly for low rupture forces.
  • To investigate the kinetic parameters of specific and nonspecific molecular bonds.

Main Methods:

  • Modeling the detection probability of rupture events as a function of probe velocity.
  • Identifying optimal probe velocities based on bond formation probability and detection limitations.
  • Analyzing experimental rupture force data from biotin-streptavidin and linear alkane bonds.

Main Results:

  • A maximum detection probability occurs at an optimal probe velocity, independent of dissociation rate.
  • The optimal velocity depends on the distance to the barrier kinetic parameter.
  • Specific bond rupture analysis aligns with previous measurements, while nonspecific bond analysis suggests heterogeneity.

Conclusions:

  • Detection probability in AFM force spectroscopy provides a viable alternative for kinetic parameter extraction.
  • The developed model allows for the determination of kinetic parameters from probe velocity dependence and detection threshold.
  • This approach offers valuable insights into molecular bond dynamics, especially for low rupture forces and heterogeneous systems.