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Updated: Jun 12, 2026
Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
Published on: January 10, 2025
Comparison between in vitro qualities of platelets washed with commercially available additive solutions and those
J Hirayama1, H Azuma, M Fujihara
1Hokkaido Red Cross Blood Center, Yamanote 2-2, Nishi-ku, Sapporo, Japan. hiraj@hokkaido.bc.jrc.or.jp
Background And Objectives:
We previously developed a novel additive solution (M-sol) with a high ability to preserve the in vitro qualities of platelets (PLTs) in washed PLTs Here, we compared the ability of M-sol with that of commercially available additive solutions (ASs) to preserve the in vitro qualities (pH, mean PLT volume, %disc, P-selectin, %hypotonic shock response and aggregation) of PLTs at a low plasma concentration.
Materials And Methods:
The platelet concentrate was divided into two equal aliquots (control group and test group). After centrifugation of both groups and removal of as much supernatant as possible, the pellet of the control group was resuspended in M-sol and those of the test group were resuspended in other ASs, and subsequently stored in polyolefin bags with agitating at 20-24 degrees C.
Results:
Compared with those stored in M-sol, the qualities of PLTs stored in PAS-B (alternative name; PAS-II or T-sol), PAS- C (alternative name; PAS-III or Intersol) or Plasma Lyte were degraded as early as 24 h after washing. The qualities of PLTs stored in PAS-D (alternative name; Composol PS) or PAS-E (alternative name; PAS-IIIM or SSP+) were comparable to that of those stored in M-sol 24 h after washing; however, the qualities had deteriorated 72 h after washing.
Conclusions:
At a low plasma concentration (5% or less), the M-sol showed a higher ability to preserve PLTs than the five ASs studied here. Although PAS-D and PAS-E are available as an AS for short-term storage of washed PLTs, M-sol is thought to be preferable for longer storage.
Insights
M-sol preserves washed platelet (PLT) quality better than five other additive solutions (ASs) at low plasma concentrations. M-sol is recommended for longer-term storage of PLTs compared to PAS-D and PAS-E.
Area of Science:
- Transfusion Medicine
- Hematology
- Biotechnology
Background:
- Platelet (PLT) quality is crucial for transfusion efficacy.
- Washed PLTs require specialized storage solutions to maintain viability.
- Novel additive solutions (ASs) are continuously being developed to improve PLT preservation.
Purpose of the Study:
- To compare the efficacy of a novel additive solution, M-sol, against commercially available ASs for preserving in vitro platelet quality.
- To evaluate PLT preservation at low plasma concentrations (≤5%).
Main Methods:
- Washed platelet concentrates were divided into control (M-sol) and test (other ASs) groups.
- Platelets were stored in polyolefin bags with agitation at 20-24°C.
- In vitro qualities assessed included pH, mean PLT volume, %disc, P-selectin, %hypotonic shock response, and aggregation.
Main Results:
- M-sol demonstrated superior preservation of PLT qualities compared to PAS-B, PAS-C, and Plasma Lyte at 24 hours.
- PAS-D and PAS-E showed comparable results to M-sol at 24 hours but deteriorated by 72 hours.
- M-sol maintained PLT quality better than the tested ASs over the study period.
Conclusions:
- M-sol exhibits enhanced ability to preserve the in vitro qualities of washed platelets at low plasma concentrations.
- While PAS-D and PAS-E are suitable for short-term storage, M-sol is preferable for extended preservation of washed platelets.
