Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Retinoblastoma cell cycling.

S Kaleta-Michaels1, D H Char, S M Kroll

  • 1Department of Ophthalmology, Francis I. Proctor Foundation, San Francisco.

Cytometry
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic copy number alterations in clear cell renal carcinoma: associations with case characteristics and mechanisms of VHL gene inactivation.

Oncogenesis·2013
Same author

PI(3) kinase is associated with a mechanism of immunoresistance in breast and prostate cancer.

Oncogene·2008
Same author

Comparative genomic hybridization.

Current protocols in human genetics·2008
Same author

Iris ring melanoma: fine needle biopsy.

The British journal of ophthalmology·2006
Same author

Prognostic relevance of 20q13 gains in sporadic colorectal cancers: a FISH analysis.

Scandinavian journal of gastroenterology·2004
Same author

20 MHz high frequency ultrasound assessment of scleral and intraocular conjunctival squamous cell carcinoma.

The British journal of ophthalmology·2002
Same journal

Measurement of the distribution of red blood cell deformability using an automated rheoscope.

Cytometry·2002
Same journal

Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

Cytometry·2002
Same journal

Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

Cytometry·2002
Same journal

Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

Cytometry·2002
Same journal

Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

Cytometry·2002
Same journal

Separation of live cells in different phases of the cell cycle for gene expression analysis.

Cytometry·2002
See all related articles

Bromodeoxyuridine (BrdUrd) measurements in retinoblastoma showed approximately 14% of viable cells in synthesis phase. Correlations between cell suspension and tissue section BrdUrd measurements were not significant.

Area of Science:

  • Ophthalmology
  • Oncology
  • Cell Biology

Background:

  • Bromodeoxyuridine (BrdUrd) is used to measure cell proliferation.
  • Accurate BrdUrd measurement can be influenced by technical and tumor factors.
  • Tumor growth fraction is difficult to assess with BrdUrd staining.

Purpose of the Study:

  • To prospectively study retinoblastoma tumor cell cycling using BrdUrd.
  • To evaluate BrdUrd measurements in cell suspensions versus tissue sections.
  • To correlate BrdUrd data with DNA content analysis.

Main Methods:

  • In vivo and in vitro BrdUrd labeling in retinoblastoma patients.
  • Microscopic evaluation of paraffin-embedded tumor sections.
  • Fluorescent activated cell sorting for cell suspensions.

Related Experiment Videos

  • Flow cytometry for DNA content analysis.

    • Main Results:

    • Approximately 14% of viable cells were in the synthesis phase.
    • No significant correlation (r = 0.48) between BrdUrd in cell suspensions and sections.
    • Most tumors had normal diploid DNA content; some showed aneuploidy.
    • No correlation found between abnormal DNA content and synthesis phase percentage.

    Conclusions:

    • Standard BrdUrd measurement techniques may have limitations in retinoblastoma.
    • Cell suspension and tissue section BrdUrd assays may not correlate.
    • DNA content abnormalities were observed but not linked to proliferation rates.