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Updated: Jun 12, 2026

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

Quantitative HPLC analysis of rat neurophysin processing.

R W Newcomb1, J J Nordmann

  • 1Békésy Laboratory of Neurobiology, University of Hawaii, 1993 East-West Road, Honolulu, HI 96822, U.S.A.

Neurochemistry International
|May 27, 2010
PubMed
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This study developed an HPLC method to quantify neuropeptides in rat neural lobes, revealing how secretory granule age relates to peptide conversion and release dynamics under varying physiological conditions.

Area of Science:

  • Neuroendocrinology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Neuropeptides exist in multiple bioactive forms, necessitating quantitative analysis of their metabolism.
  • Understanding variations in neuropeptide forms is crucial for comprehending neural lobe function.

Purpose of the Study:

  • To develop a sensitive HPLC method for quantifying major neural lobe peptides in Wistar rats.
  • To use peptide conversion as an indirect measure of secretory granule age under various conditions.
  • To investigate the relationship between neuropeptide storage, release, and secretory demand.

Main Methods:

  • Developed a sensitive HPLC procedure with on-line fluorescamine reaction for peptide quantification.
  • Defined peptide peaks using amino acid analysis, carboxypeptidase digestion, gel electrophoresis, gel filtration, and Edman degradation.

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Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

Related Experiment Videos

Last Updated: Jun 12, 2026

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

  • Quantified oxytocin neurophysin conversion to assess secretory granule age in different physiological states (age, dehydration, osmotic stress) and isolated granules.

    • Main Results:

    • Oxytocin neurophysin conversion increased in larger animals, denser granules, and early dehydration, but decreased in smaller animals, later dehydration, chronic osmotic stress, and denser granules.
    • High potassium perfusates showed minimal change in peptide conversion.
    • Peptide content in axon terminals and swellings is relatively homogeneous, suggesting fast granule transfer rates.

    Conclusions:

    • Secretory granule dynamics, including transfer rates between axonal compartments, are critical for regulating secreted peptide content.
    • The developed HPLC method provides a sensitive tool for quantitative neuropeptide analysis.
    • Granule movement significantly influences how peptide secretion responds to changing demands.