Related Experiment Video
Updated: Jun 12, 2026
10:13
Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
Published on: June 9, 2017
GPCR-OKB: the G Protein Coupled Receptor Oligomer Knowledge Base
George Khelashvili1, Kevin Dorff, Jufang Shan
1Department of Physiology & Biophysics, Weill Medical College of Cornell University, New York, NY, USA.
Bioinformatics (Oxford, England)
|May 27, 2010
Summary
The GPCR-Oligomerization Knowledge Base (GPCR-OKB) organizes G Protein Coupled Receptor (GPCR) dimer and oligomer data. This system aids researchers by providing searchable literature-derived information on GPCR oligomers.
Area of Science:
- Pharmacology
- Bioinformatics
- Structural Biology
Background:
- G Protein Coupled Receptors (GPCRs) form dimers and oligomers.
- An increasing volume of data on GPCR oligomerization necessitates structured organization.
Purpose of the Study:
- To develop an electronic system for organizing and accessing GPCR oligomerization data.
- To create a searchable knowledge base for GPCR dimer and oligomer information.
Main Methods:
- Developed the GPCR-Oligomerization Knowledge Base (GPCR-OKB) based on a community-derived ontology.
- Manually curated GPCR oligomer data from scientific literature.
- Integrated GPCR-OKB with GPCRDB for comprehensive data correlation.
Main Results:
- GPCR-OKB provides a structured platform for browsing and searching GPCR oligomer data.
- The knowledge base facilitates the study of GPCR protomers and their oligomeric states.
- Data is derived from manual literature review, ensuring curated information.
Conclusions:
- GPCR-OKB effectively organizes the expanding knowledge of GPCR oligomerization.
- The system supports researchers in understanding GPCR dimer and oligomer functions.
- Seamless integration with GPCRDB enhances the utility of GPCR-OKB for correlative studies.
More Related Videos
Related Concept Videos
01:15G Protein-coupled Receptors
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
01:15G Protein-coupled Receptors
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
01:21G-protein Coupled Receptors
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
01:21G-protein Coupled Receptors
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
01:30Transducer Mechanism: G Protein–Coupled Receptors
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical, 7TM, or...
GPCRs are also called heptahelical, 7TM, or...
01:09GPCRs Regulate Adenylyl Cylase Activity
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of cells.
Two...
Two...