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Updated: Jun 12, 2026

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Published on: March 22, 2017

Decoded calreticulin-deficient embryonic stem cell transcriptome resolves latent cardiophenotype.

Randolph S Faustino¹, Anca Chiriac, Nicolas J Niederlander

¹Marriott Heart Disease Research Program, Division of Cardiovascular Diseases, Departments of Medicine, Molecular Pharmacology and Experimental Therapeutics, and Medical Genetics, Mayo Clinic, Rochester, Minnesota 55905, USA.

Stem Cells (Dayton, Ohio)

|May 28, 2010

Summary

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Calreticulin deficiency in embryonic stem cells disrupts cellular functions and leads to cardiac defects, including a ventricular septal defect. Early transcriptome analysis can predict these developmental abnormalities before symptoms appear.

Area of Science:

Systems Biology
Developmental Biology
Genomics

Background:

Genomic alterations at the pluripotent stage can cause developmental abnormalities.
Early identification of molecular signatures can predict latent phenotypes.
Understanding calreticulin's role in stem cell development is crucial.

Purpose of the Study:

To assess the impact of calreticulin-deficient embryonic stem cell transcriptomes on cellular functions and physiological systems.
To identify transcriptome landscapes predicting developmental aberrations.
To investigate the role of calreticulin in early cardiac development.

Main Methods:

Bioinformatic analysis of calreticulin-null embryonic stem cell transcriptomes.
Network cartography of undifferentiated stem cell transcripts.

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Last Updated: Jun 12, 2026

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

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Published on: March 22, 2017

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

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Published on: December 28, 2011

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

In vitro differentiation of stem cells into cardiac cells.

Magnetic resonance imaging and histopathology.

Main Results:

Calreticulin deficiency disrupted transcriptome dynamics and re-prioritized cellular functions.
Network analysis predicted cardiac manifestations.
Calreticulin-deficient cardiac cells showed disorganized sarcomerogenesis, mitochondrial issues, and cytoarchitectural defects.
A ventricular septal defect was detected in vivo.

Conclusions:

Bioinformatic analysis of calreticulin-deficient transcriptomes at the pluripotent stage can predict susceptibility to abnormal cardiophenotypes.
Calreticulin is essential for normal stem cell function and cardiac development.
Systems biology approaches can anticipate developmental abnormalities through transcriptome surveillance.