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Related Experiment Videos

NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.

A Acheson1, J L Sunshine, U Rutishauser

  • 1Department of Anatomy and Cell Biology, University of Alberta, Edmonton, Canada.

The Journal of Cell Biology
|July 1, 1991
PubMed
Summary
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Polysialic acid (PSA) on neural cell adhesion molecule (NCAM) regulates cell interactions. Removing PSA enhances cell-cell and cell-substrate adhesion, impacting neural development.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • The polysialic acid (PSA) moiety of neural cell adhesion molecule (NCAM) is known to modulate cell interactions.
  • Previous hypotheses suggest PSA influences membrane-membrane apposition and regulates contact-dependent cell interactions.

Purpose of the Study:

  • To provide direct evidence that PSA on the cell surface affects both cell-cell and cell-substrate interactions.
  • To investigate the role of PSA in neural development, specifically axon bundling.

Main Methods:

  • Utilized cell and tissue culture models.
  • Employed a neuroblastoma/sensory neuron cell hybrid system.
  • Used a specific neuraminidase (endo-N) to remove PSA.
  • Studied embryonic spinal cord axon bundling.

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Main Results:

  • Removal of PSA using endo-N augmented cell-cell aggregation mediated by L1 cell adhesion molecule.
  • Removal of PSA increased cell attachment to various tissue culture substrates.
  • Removal of PSA led to pronounced defasciculation in embryonic spinal cord axon bundling, suggesting increased cell-substrate interaction.

Conclusions:

  • The presence of PSA on the cell surface significantly affects cell-cell and cell-substrate interactions.
  • PSA acts as a regulator of cell adhesion beyond the intrinsic binding function of the NCAM polypeptide.
  • Changes in PSA levels can influence neural development processes like axon fasciculation.