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Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
Published on: November 18, 2016
VEGF expression in dog mastocytoma
O Mederle1, Narcisa Mederle, Elena Viorica Bocan
1Universitatea de Medicină Si Farmacie V. Babes Timişoara, Facultatea de Medicină, Catedra de Histologie şi Citologie.
Summary
Vascular endothelial growth factor (VEGF) expression in canine mastocytoma tumor cells correlates with aggressive behavior and poor prognosis, indicating its role in tumor progression.
Area of Science:
- Veterinary Pathology
- Oncology
- Immunohistochemistry
Background:
- Mastocytomas in dogs show variable biologic behavior, ranging from benign to aggressive with metastasis.
- Vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis and a potential autocrine growth factor in malignancies.
Purpose of the Study:
- To investigate the expression of VEGF in canine cutaneous mastocytoma.
- To correlate VEGF expression with other markers like mast cell tryptase, CD117, and vimentin.
Main Methods:
- Immunohistochemistry was used to detect VEGF expression in canine cutaneous mast cell tumors.
- VEGF expression was analyzed alongside mast cell tryptase, CD117, and vimentin.
Main Results:
- Tumor cells demonstrated positive VEGF expression with a heterogeneous, cytoplasmic pattern of moderate to strong intensity.
- Inflammatory cells within the tumor microenvironment were negative for VEGF.
- VEGF-expressing tumor cells were predominantly located near the proliferation front.
Conclusions:
- VEGF secretion by mastocytoma cells is associated with aggressive tumor behavior.
- VEGF expression in canine mastocytoma correlates with an unfavorable prognosis, suggesting its role in tumor progression and metastasis.
