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Updated: Jun 12, 2026

Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
[Study on molecular characteristics regarding DNA genotype of Mycobacterium tuberculosis clinical strains in
Yun-feng Deng1, Yan-an Zhang, Jian-li Zheng
1Shandong Provincial Chest Hospital, Jinan 250013, China. yfdeng@126.com
Objective:
To establish the molecular characteristics of Mycobacterium tuberculosis and on factors influencing the recent transmission of drug resistant isolates in Shandong.
Methods:
Mycobacterium tuberculosis isolated from active pulmonary tuberculosis patients of 13 counties were genotyped by mycobacterial interspersed repetitive units (MIRU) methods.
Results:
12 loci of MIRU were detected in 558 isolates and a total of 143 MIRU patterns were confirmed. 66 isolates had distinct patterns, and 481 (86.2%) strains were in clusters. Shandong cluster included 177 strains with 74.6% of the isolates belonged to Beijing family. The recent transmission index of multi-drug resistance strains was in lower level, comparing to the susceptible strains.
Conclusion:
Our results showed that the Shandong cluster isolates had capacities of facilitating person-to-person transmission and high level of drug resistance.
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Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
