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Related Experiment Video

Updated: Jun 12, 2026

Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

Estimating prion concentration in fluids and tissues by quantitative PMCA.

Baian Chen1, Rodrigo Morales, Marcelo A Barria

  • 1Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, University of Texas Houston Medical School, Houston, Texas, USA.

Nature Methods
|June 1, 2010
PubMed
Summary

This study introduces a quantitative protein misfolding cyclic amplification (PMCA) method to measure prion concentration in biological samples. The technique accurately quantified misfolded prion protein (PrPSc) in various tissues from hamsters with scrapie.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Infectious Diseases

Background:

  • Prions are infectious proteins causing fatal neurodegenerative diseases.
  • Protein misfolding cyclic amplification (PMCA) is a sensitive method for prion detection.
  • Accurate quantification of prions is crucial for understanding disease pathogenesis and transmission.

Purpose of the Study:

  • To develop and validate a quantitative PMCA procedure.
  • To determine the concentration of misfolded prion protein (PrPSc) in biological samples.
  • To assess prion levels in different tissues of scrapie-affected hamsters.

Main Methods:

  • Development of a quantitative protein misfolding cyclic amplification (PMCA) assay.
  • Application of the assay to biological samples from scrapie-affected hamsters.

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Last Updated: Jun 12, 2026

Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

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  • Measurement of PrPSc concentration in brain, spleen, blood, and urine.

    • Main Results:

    • A sensitive quantitative PMCA procedure was established.
    • The method successfully measured very low prion concentrations.
    • PrPSc quantities were determined in the brain, spleen, blood, and urine of infected hamsters.

    Conclusions:

    • Quantitative PMCA enables precise measurement of prion levels in biological samples.
    • This method is valuable for studying prion diseases and their distribution.
    • The findings contribute to a better understanding of prion pathogenesis.