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Plastics, endotoxins, and the Limulus amebocyte lysate test
P F Roslansky1, M E Dawson, T J Novitsky
1Associates of Cape Cod, Inc., Falmouth, Massachusetts.
Journal of Parenteral Science and Technology : a Publication of the Parenteral Drug Association
|March 1, 1991
Summary
Choosing the right laboratory tubes is crucial for accurate Limulus amebocyte lysate (LAL) testing. Some polypropylene and polystyrene tubes interfere with endotoxin detection, while others perform comparably to glass.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Microbiology
Background:
- The Limulus amebocyte lysate (LAL) test is a sensitive method for detecting bacterial endotoxins.
- Accurate LAL test results depend on the quality and inertness of laboratory consumables.
- Polypropylene and polystyrene tubes are commonly used but their suitability for LAL testing requires evaluation.
Purpose of the Study:
- To assess the compatibility of various polypropylene and polystyrene tubes with the LAL test.
- To identify potential sources of interference or contamination in commonly used laboratory tubes.
Main Methods:
- Multiple brands of polypropylene and polystyrene tubes were tested.
- Tubes were evaluated for endotoxin contamination and their effect on LAL test performance.
- Endotoxin storage and dilution water compatibility were assessed.
Main Results:
- Polypropylene tubes showed higher endotoxin contamination compared to polystyrene.
- One polypropylene tube type contained an inhibitor affecting the LAL test.
- Some polystyrene tubes enhanced the LAL test, while others were suitable for endotoxin storage and dilution.
Conclusions:
- Tube material and brand significantly impact LAL test reliability.
- Careful selection of polypropylene and polystyrene tubes is necessary for accurate endotoxin detection.
- Glass tubes remain a reliable standard, but suitable plastic alternatives exist.