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Updated: Jun 12, 2026
09:42
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
Immunization and fusion protocols for hybridoma production
1Department of Experimental Immunobiology, Wellcome Research Laboratories, Beckenham, Kent.
Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2010
Summary
The Kohler and Milstein technique enables the production of monoclonal antibodies by immortalizing antibody-producing plasma cells. This method provides a consistent supply of specific antibodies for diverse biological applications.
Area of Science:
- Immunology
- Biotechnology
- Cell Biology
Background:
- The Kohler and Milstein technique revolutionized antibody production.
- It involves immortalizing antibody-secreting plasma cells for continuous antibody supply.
- Monoclonal antibodies offer high specificity compared to polyclonal antisera.
Purpose of the Study:
- To describe the principles and advantages of monoclonal antibody production using the Kohler and Milstein technique.
- To highlight the utility of monoclonal antibodies in various biological research areas.
- To discuss the current limitations, particularly regarding human monoclonal antibody generation.
Main Methods:
- Hybridoma technology: fusing plasma cells with myeloma cells to create immortal cell lines.
- Selection and cloning of hybridoma cells that secrete antibodies against a specific antigen.
- Culturing hybridoma cell lines to produce large quantities of monoclonal antibodies.
Main Results:
- Established hybridoma cell lines provide an inexhaustible supply of monoclonal antibodies.
- Monoclonal antibodies possess consistent and reproducible properties.
- Production is feasible without extensive immunogen purification or cross-adsorption.
Conclusions:
- The Kohler and Milstein technique is a cornerstone for producing specific monoclonal antibodies.
- Hybridoma technology offers significant advantages in antibody production efficiency and specificity.
- Further advancements are needed to overcome challenges in producing human monoclonal antibodies for therapeutic use.
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