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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
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Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
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Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Updated: Jun 12, 2026

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example
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Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Published on: December 3, 2014

Rocket immunoelectrophoresis.

J M Walker1

  • 1School of Biological and Environmental Sciences, The Hatfield Polytechnic, Hatfield, Hertfordshire, England.

Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2010
PubMed
Summary
This summary is machine-generated.

Rocket immunoelectrophoresis provides a fast and reliable way to measure specific protein concentrations in mixtures. This electroimmunoassay technique uses antigen-antibody reactions in an agarose gel to quantify proteins accurately.

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Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions
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Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

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Last Updated: Jun 12, 2026

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example
12:44

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Published on: December 3, 2014

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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

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Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

Published on: January 5, 2024

Area of Science:

  • Biochemistry
  • Immunology
  • Analytical Chemistry

Background:

  • Quantifying specific proteins in complex mixtures is crucial for various biological and diagnostic applications.
  • Traditional methods may lack specificity or efficiency in protein concentration determination.

Purpose of the Study:

  • To describe the principle and application of Rocket immunoelectrophoresis for protein quantification.
  • To highlight the method's simplicity, speed, and reproducibility.

Main Methods:

  • Rocket immunoelectrophoresis (electroimmunoassay) involves electrophoresing antigens into an antibody-containing agarose gel.
  • Precipitation 'rockets' form as antigen-antibody complexes reach equivalence.
  • Comparison with known standards allows for concentration determination.

Main Results:

  • The method yields a characteristic precipitation 'rocket' pattern.
  • The height of the rocket is proportional to the antigen concentration.
  • Accurate protein quantification is achieved through comparison with serial dilutions.

Conclusions:

  • Rocket immunoelectrophoresis is a robust and efficient technique for specific protein quantification.
  • Its simplicity and reproducibility make it valuable in various scientific fields.
  • The method facilitates precise measurement of protein concentrations in biological samples.