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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

Methods of Documentation III: PIE

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Problem-intervention-evaluation (PIE) is a systematic approach to documentation used in healthcare settings for clinical decision-making and patient care planning. It is a structured approach to organizing patient data based on problems, interventions, and evaluations. Here's a breakdown of its key features and considerations:

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Related Experiment Video

Updated: Jun 12, 2026

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

Paper-based ELISA

Chao-Min Cheng¹, Andres W Martinez, Jinlong Gong

¹Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

Angewandte Chemie (International Ed. in English)

|June 1, 2010

Summary

No abstract available in PubMed .

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