Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Phenotypic similarity of adverse drug reactions and disease phenotypes is a bridge to mechanistic discovery.

npj drug discovery·2026

Same author

Origin of sexual dimorphism in osteoarthritis risk: the impact of pregnancy and parental care.

BMC public health·2026

Same author

Drugst.One DREAM-Drug repurposing through expert annotation and modification.

British journal of pharmacology·2026

Same author

Older adults with osteoarthritis show lower functional performance compared to those with diabetes or hypertension: Evidence from the SHARE dataset.

Osteoarthritis and cartilage open·2026

Same author

Leveraging human genetic variation to therapeutically target hundreds of genes with dominant & dispensable disease alleles.

medRxiv : the preprint server for health sciences·2026

Same author

Estimating motion artifacts of HR-pQCT scans using automatically derived standard parameters of bone structure- and density-quantification.

Bone·2026

Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026

Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026

Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026

Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026

Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026

Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

AltAnalyze and DomainGraph: analyzing and visualizing exon expression data.

Dorothea Emig¹, Nathan Salomonis, Jan Baumbach

¹Max Planck Institute for Informatics, 66123 Saarbrücken, Germany. demig@mpi-inf.mpg.de

Nucleic Acids Research

|June 2, 2010

Summary

This summary is machine-generated.

We developed new software to analyze alternative splicing, a process that increases protein diversity. This tool helps visualize how splicing affects protein domains and biological pathways, aiding in understanding its functional impact.

More Related Videos

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

Published on: July 29, 2022

Mapping Alzheimer's Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration

Mapping Alzheimer's Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration

Published on: January 9, 2020

Related Experiment Videos

Last Updated: Jun 12, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

Published on: July 29, 2022

Mapping Alzheimer's Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration

Mapping Alzheimer's Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration

Published on: January 9, 2020

Area of Science:

Genomics and Bioinformatics
Molecular Biology

Background:

Alternative splicing significantly expands protein diversity.
The functional consequences of alternative splicing remain largely unexplored.

Purpose of the Study:

To introduce a novel software workflow for analyzing and visualizing alternative splicing data.
To provide an end-to-end solution for interpreting the functional effects of alternative splicing.

Main Methods:

Developed AltAnalyze (open-source application) and DomainGraph (Cytoscape plugin).
Utilized Affymetrix Exon and Gene Arrays for data analysis.
Incorporated rigorous statistical methods like FIRMA, MiDAS, and DABG filtering.

Main Results:

Enabled analysis of alternative splicing at protein, domain, and microRNA binding site levels.
Facilitated visualization of alternative splicing effects on protein domains and microRNA binding sites.
Integrated visualization of affected pathways and gene/protein interaction networks.

Conclusions:

The software offers an intuitive, comprehensive solution for alternative splicing analysis.
Enables straightforward identification of potential biological effects of alternative splicing.
Accessible tools require no prior programming or exon array analysis knowledge.