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Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

Novel biocompatible DNA gel particles.

M Carmen Morán¹, M Rosa Infante, M Graça Miguel

¹Chemistry Department, Rua Larga, Coimbra University, 3004-535 Coimbra, Portugal. mcarmen@qui.uc.pt

Langmuir : the ACS Journal of Surfaces and Colloids

|June 3, 2010

Summary

Novel biocompatible DNA gel particles were created using amino acid-based surfactants. Stronger interactions were observed with double-charged surfactants and single-stranded DNA, influencing controlled release kinetics.

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Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

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Published on: August 6, 2019

Area of Science:

Biomaterials Science
Supramolecular Chemistry
Nucleic Acid Chemistry

Background:

Amino acid-based surfactants offer biocompatibility for novel drug delivery systems.
Controlled DNA encapsulation and release are crucial for gene therapy and diagnostics.
Understanding surfactant-nucleic acid interactions is key to designing effective delivery vehicles.

Purpose of the Study:

To investigate the formation and properties of DNA gel particles using two distinct amino acid-based surfactants.
To analyze the influence of surfactant charge and DNA secondary structure on DNA entrapment and release.
To elucidate the microstructure and interaction mechanisms within these DNA-surfactant complexes.

Main Methods:

Preparation of DNA gel particles by mixing DNA (ssDNA/dsDNA) with arginine-N-lauroyl amide dihydrochloride (ALA) and N(alpha)-lauroyl-arginine-methyl ester hydrochloride (LAM).
Characterization of DNA entrapment, swelling/deswelling behavior, and DNA release kinetics.
Small-angle X-ray scattering (SAXS) for microstructure analysis.
Agarose gel electrophoresis to confirm DNA complexation and neutralization.

Main Results:

ALA exhibited stronger interaction with DNA than LAM, attributed to its double-charged headgroup.
Single-stranded DNA (ssDNA) showed stronger interaction with surfactants than double-stranded DNA (dsDNA), highlighting the role of hydrophobic interactions.
SAXS data indicated hexagonal packing in the complexes, with shorter lattice parameters correlating to stronger interactions and slower DNA release.
Agarose gel electrophoresis confirmed DNA complexation and neutralization within the gel particles.

Conclusions:

Amino acid-based surfactants can form biocompatible DNA gel particles for controlled DNA delivery.
Surfactant charge and DNA secondary structure significantly modulate DNA-surfactant interactions and release profiles.
The observed hexagonal packing and lattice parameter provide insights into the structural basis of controlled DNA release.