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Detecting DNA folding with nanocapillaries.

Lorenz J Steinbock1, Oliver Otto, Catalin Chimerel

  • 1Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom.

Nano Letters
|June 3, 2010
PubMed
Summary

Researchers detected DNA folding states using tiny glass nanocapillaries and electrical signals. This label-free method offers a new way to analyze DNA in solution, similar to silicon nanopores.

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Area of Science:

  • Biophysics
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Analyzing the folding state of DNA is crucial for understanding its function.
  • Existing methods for DNA analysis can be complex or require labeling.
  • Nanopore technology offers potential for label-free molecular analysis.

Purpose of the Study:

  • To demonstrate the detection of double-stranded DNA folding states using nanocapillaries.
  • To investigate the translocation of DNA through nanocapillaries driven by electrophoresis.
  • To evaluate nanocapillaries as an alternative to solid-state nanopores for DNA analysis.

Main Methods:

  • Fabrication of glass nanocapillaries with diameters down to 45 nm.
  • Resistive pulse technique to monitor ionic current changes.

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  • Electrophoretic translocation of lambda-DNA through nanocapillaries.

    • Main Results:

    • Successfully detected the folding state of single lambda-DNA molecules based on ionic current changes.
    • Demonstrated that glass nanocapillaries can be reliably fabricated for DNA analysis.
    • Observed DNA translocation driven by electrophoretic forces.

    Conclusions:

    • Nanocapillaries are suitable for label-free analysis of DNA in aqueous solutions.
    • Glass nanocapillaries provide a viable alternative to silicon-based solid-state nanopores.
    • The resistive pulse technique in nanocapillaries can distinguish DNA folding states.