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Related Concept Videos

The Significance of Membrane Transport01:44

The Significance of Membrane Transport

The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
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Related Experiment Video

Updated: Jun 19, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

Label Type Influence on DNA Translocation Velocity in Solid-State Nanopores.

Simon Brauburger1, Thieme Schmidt1, Filip Bošković1

  • 1Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0US, U.K.

ACS Nano
|June 17, 2026
PubMed
Summary
This summary is machine-generated.

Dense molecular labeling on DNA carriers minimally impacts translocation time in solid-state nanopores. This finding supports applications like DNA data storage and molecular barcoding without needing label-specific corrections.

Keywords:
DNA nanotechnologyMolecular labelingNanopore sensingPolymer translocationSingle-molecule biophysicsSolid-state nanoporesTranslocation dynamics

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Monitoring Protein Adsorption with Solid-state Nanopores
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Last Updated: Jun 19, 2026

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Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Biophysics

Background:

  • Solid-state nanopores facilitate single-molecule detection of nucleic acids, enabling precise localization of molecular labels on DNA carriers.
  • Applications include molecular barcoding, protein mapping, and DNA data storage, often assuming minimal perturbation by these labels.
  • The impact of dense molecular labeling on DNA translocation dynamics and positional readout accuracy is not well-quantified.

Purpose of the Study:

  • To systematically quantify the effect of dense molecular labeling on DNA carrier translocation times in solid-state nanopores.
  • To assess the influence of different label types (DNA nanostructures, streptavidin, PEG) on translocation dynamics.
  • To determine if label-specific velocity corrections are necessary for applications like molecular barcoding.

Main Methods:

  • Utilized glass nanopipettes (8-12 nm diameter) as solid-state nanopores.
  • Employed 7.2 kbp DNA carriers with up to 60 labels of various types (DNA nanostructures, streptavidin, PEG).
  • Systematically measured and analyzed translocation times and label-associated event timings.

Main Results:

  • Despite significant increases in mass (up to 83%) and charge (up to 23%), all tested labels caused modest changes (<±15%) in global translocation time, below measurement variability (∼20%).
  • The velocity profile during translocation remained consistent across label types.
  • A substantial portion (40-70%) of the translocation time shift occurred in labeled regions, though these comprised only 20% of the carrier.

Conclusions:

  • Dense molecular labeling has a minimal impact on overall DNA carrier translocation times in nanopores.
  • Relative label positions are largely unaffected, preserving positional readout accuracy.
  • Molecular barcoding and protein-positioning assays can generally proceed without label-specific velocity corrections under studied conditions.