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Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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Improved interferometric tracking of trapped particles using two frequency-detuned beams.

Lars Friedrich1, Alexander Rohrbach

  • 1Laboratory for Bio- and Nano-Photonics, Institute for Microsystem Technology (IMTEK) University of Freiburg, Germany.

Optics Letters
|June 3, 2010
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Summary

Precise optical tweezer particle tracking is improved using a second frequency-detuned focus to extend axial detection range. Noise in axial signals is also reduced with an additional detector for more reliable measurements.

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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

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Published on: October 1, 2014

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Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
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Published on: April 22, 2013

Area of Science:

  • Optical physics
  • Nanotechnology
  • Biophysics

Background:

  • Precise particle tracking is crucial for optical tweezer applications.
  • Backfocal-plane interferometry offers high speed and accuracy but is limited by particle displacement.
  • Nonlinear detector response, particularly for positive axial displacements, can cause tracking errors.

Purpose of the Study:

  • To enhance the linear detection range for axial particle displacements in optical tweezers.
  • To improve the accuracy and reliability of particle tracking beyond the conventional limits.
  • To reduce noise in axial position measurements.

Main Methods:

  • Utilized a second frequency-detuned tracking focus generated from the same laser as the optical trap.
  • Implemented a dual-detector system to process signals from the tracking foci.
  • Analyzed the detector response and signal-to-noise ratio for axial displacement measurements.

Main Results:

  • Extended the linear detection range in the axial direction by a factor of 2 to 4.
  • Demonstrated significantly reduced noise in the axial position signal.
  • Achieved more accurate and reliable tracking of trapped particles, especially for larger axial displacements.

Conclusions:

  • The proposed method effectively overcomes limitations of standard backfocal-plane interferometry for axial tracking.
  • Employing a frequency-detuned secondary focus and dual detectors enhances optical tweezer tracking performance.
  • This technique offers a robust solution for precise particle manipulation and measurement in various scientific fields.