Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thermal fluctuations of the lipid membrane determine particle uptake into Giant Unilamellar Vesicles.

Nature communications·2023
Same author

100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales.

Nature communications·2022
Same author

Towards non-blind optical tweezing by finding 3D refractive index changes through off-focus interferometric tracking.

Nature communications·2021
Same author

Deep-ROCS: from speckle patterns to superior-resolved images by deep learning in rotating coherent scattering microscopy.

Optics express·2021
Same author

IgE antibody repertoire in nasal secretions of children and adults with seasonal allergic rhinitis: A molecular analysis.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2019
Same author

Strong cytoskeleton activity on millisecond timescales upon particle binding revealed by ROCS microscopy.

Cytoskeleton (Hoboken, N.J.)·2018
Same journal

Sodium-Based Battery Component Design: Imitating Lithium or Forging New Paths?

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Enhancing Birefringence of Sulphates by Polarity Modification in Planar Cations.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

In Situ Atomic-Scale Observation of Preferential Premelting at Oxide Crystal Defects.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Thickness-Dependent Semiconductor-Metal Transition in Two-Dimensional Nonlayered Magnetic CuCo<sub>2</sub>S<sub>4</sub>.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Programmable Control Over Radical and Non‑Radical Pathways in Fenton‑Like Catalysis via Carbon‑Encapsulated Iron Nanoreactors.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Self-Powered MXene@Perovskite Thermoelectric Skin for Multimodal Mid-Infrared Sensing and Human Signal Recognition.

Small (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

11.5K

Making Hidden Cell Particle Interactions Visible by Thermal Noise Frequency Decomposition.

Felix Jünger1, Alexander Rohrbach1,2

  • 1Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 102, 79110, Freiburg, Germany.

Small (Weinheim an Der Bergstrasse, Germany)
|June 20, 2023
PubMed
Summary
This summary is machine-generated.

High-speed tracking reveals hidden cellular interactions by analyzing particle motion. This method uncovers invisible binding behaviors and measures cell properties like stiffness and glycocalyx extent.

Keywords:
filopodiafrequency decompositionglycocalyxparticle trackingthermal fluctuationsvisco-elasticity

More Related Videos

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

11.4K
Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
08:17

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

1.9K

Related Experiment Videos

Last Updated: Jun 22, 2026

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

11.5K
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

11.4K
Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
08:17

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

1.9K

Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Thermal noise influences cellular structures, bacteria, and viruses across various scales.
  • Particle interactions with the cellular environment can be subtle and difficult to detect, leading to misunderstandings of binding behavior.

Purpose of the Study:

  • To demonstrate a method for visualizing previously hidden particle interactions at the cell periphery.
  • To analyze particle fluctuations and viscoelastic properties of cellular structures.

Main Methods:

  • Utilizing MHz interferometric thermal noise tracking and frequency decomposition.
  • Analyzing particle fluctuations in radial and lateral directions using a viscoelastic modulus G(ω,tex).
  • Employing a spectral update rate of 0.5 s to capture dynamic changes.

Main Results:

  • Revealed a similar, frequency-dependent viscoelastic response across different cell types.
  • Successfully detected the stiffening of macrophage filopodia tips within 2 s.
  • Quantified the extent and stiffness of the cell glycocalyx, which is typically invisible on longer timescales.

Conclusions:

  • High-speed particle motion tracking can uncover hidden cellular interactions and binding behaviors.
  • The developed method provides insights into molecular-scale elasticity and damping within cells.
  • This technique offers a novel approach to studying dynamic cellular processes and interactions.