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Preparation of DNA and RNA fragments containing guanine N(2)-thioalkyl tethers
Xiaorong Hou1, Gang Wang, Barbara L Gaffney
1Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
Current Protocols in Nucleic Acid Chemistry
|June 3, 2010
Abstract:
This unit describes procedures for preparation of deoxyguanosine and guanosine derivatives in which the guanine N(2) contains a thiopropyl tether, protected as a tert-butyl disulfide. After incorporation into a DNA or RNA fragment, this tether allows site-specific cross-linking to a thiol of a protein or another nucleic acid.
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