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Published on: November 11, 2022
[Study on fingerprints spectrum of Lophatherum gracile by HPLC]
1Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. 15840951126@139.com
Objective:
To establish the fingerprint spectrum of Lophatherum gracile by HPLC.
Methods:
The samples were separated on Agilent Eclipse XDB- C18 column (150 mm x 4.6 mm, 5 microm) with methanol (A) -3% acetic acid (B) as the mobile phase at a flow rate of 1.0 mL/min at 30 centigrades, adopting the gradient elution process with switching the Wavelength.
Results:
15 samples of different origin Lophatherum gracile Brongn were detected and 16 peaks in the chromatogram were common, there was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine.
Conclusion:
The method is accurate, reliable and the fingerprint spectrum provides more information for controlling the quality of Lophatherum gracile.
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