Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Postsynthetic modification of N-heterocyclic diazoolefins <i>via</i> backbone metallation.

Dalton transactions (Cambridge, England : 2003)·2026

Same author

Bent N-Heterocyclic Allenes From a Well-Defined Titanium Vinylidene Complex.

Angewandte Chemie (International ed. in English)·2026

Same author

A Metallosupramolecular Receptor for Squaraine Dyes Enabling Ultrafast Dark Resonance Energy Transfer.

Angewandte Chemie (International ed. in English)·2026

Same author

Transition Metal Complexes with <i>N</i>-Heterocyclic Vinylidene Ligands.

Chimia·2025

Same author

Wide variability in the stability of Pd<sub>6</sub>L<sub><i>x</i></sub>-type coordination cages.

Dalton transactions (Cambridge, England : 2003)·2025

Same author

Covalent capture of nitrous oxide by phosphanides.

Chemical communications (Cambridge, England)·2025

Same journal

Editorial.

Journal of combinatorial chemistry·2016

Same journal

2000 Reviewers.

Journal of combinatorial chemistry·2016

Same journal

Editorial.

Journal of combinatorial chemistry·2016

Same journal

Editorial.

Journal of combinatorial chemistry·2016

Same journal

Editorial.

Journal of combinatorial chemistry·2016

Same journal

Editorial.

Journal of combinatorial chemistry·2016

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

Pattern-based sensing with metal-dye complexes: sensor arrays versus dynamic combinatorial libraries.

Sébastien Rochat¹, Kay Severin

¹Institut des Sciences et Ingenierie Chimiques, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland.

Journal of Combinatorial Chemistry

|June 4, 2010

Summary

This summary is machine-generated.

New colorimetric sensors using metal-dye complexes effectively detect peptides. Dynamic combinatorial libraries, especially those with intermediate complexity, showed superior performance over sensor arrays for peptide differentiation.

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Related Experiment Videos

Last Updated: Jun 12, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Area of Science:

Analytical Chemistry
Chemical Sensing
Spectroscopy

Background:

Colorimetric sensors offer a promising avenue for peptide detection.
Metal-dye complexes are utilized for developing novel sensing platforms.
Dynamic combinatorial libraries (DCLs) and sensor arrays are two distinct approaches for sensor design.

Purpose of the Study:

To develop and evaluate colorimetric sensors for peptide detection using metal-dye complexes.
To compare the efficacy of sensors based on dynamic combinatorial libraries versus sensor arrays.
To identify optimal sensor design parameters for differentiating various peptides.

Main Methods:

Generation of colorimetric sensors by mixing dyes (Methyl Calcein Blue, Arsenazo I, Xylenol Orange) with metal salts (CuCl2, NiCl2).
Two sensor design strategies: (1) one-pot dynamic combinatorial libraries of metal-dye complexes, and (2) sensor arrays of six different metal-dye combinations.
Analysis of optical responses via spectral changes and multivariate analysis for differentiating 13 di- and tripeptides.

Main Results:

Sensors based on dynamic combinatorial libraries generally outperformed sensor arrays in differentiating peptides.
Intermediate complexity libraries demonstrated the best sensing performance.
The study successfully differentiated 13 different di- and tripeptides using the developed sensors.

Conclusions:

Dynamic combinatorial libraries represent a superior strategy for developing colorimetric peptide sensors compared to traditional sensor arrays.
Optimizing library complexity is crucial for achieving high sensitivity and selectivity in peptide sensing.
These findings advance the development of efficient and selective colorimetric methods for peptide analysis.