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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Cell Cycle Control System01:28

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The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

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The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

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The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...

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Updated: Jun 12, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

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Published on: May 3, 2018

Hsp90 phosphorylation, Wee1 and the cell cycle.

Mehdi Mollapour1, Shinji Tsutsumi, Len Neckers

  • 1Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.

Cell Cycle (Georgetown, Tex.)
|June 4, 2010
PubMed
Summary
This summary is machine-generated.

Heat Shock Protein 90 (Hsp90) phosphorylation by Wee1 regulates cell cycle progression. This modification is crucial for Hsp90 function, Wee1 stability, and cancer cell sensitivity to Hsp90 inhibitors.

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Last Updated: Jun 12, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Heat Shock Protein 90 (Hsp90) is a vital molecular chaperone in eukaryotic cells.
  • Hsp90 inhibition is a promising cancer therapy strategy, with many inhibitors in clinical trials.
  • Understanding factors modulating Hsp90 inhibitor efficacy is critical for clinical success.

Purpose of the Study:

  • To investigate the role of Hsp90 phosphorylation by Wee1 in cell cycle regulation.
  • To determine the impact of Hsp90 phosphorylation on Hsp90-Wee1 interactions and Wee1 stability.
  • To assess the consequences of non-phosphorylatable Hsp90 on cell division and checkpoint control.

Main Methods:

  • Utilized yeast models expressing wild-type and non-phosphorylatable Hsp90 variants (yHsp90-Y24F).
  • Examined cell cycle progression, nuclear division, and G2/M checkpoint function.
  • Assessed Hsp90-Wee1 association and Wee1 protein stability.

Main Results:

  • Tyrosine phosphorylation of Hsp90 by Wee1 is essential for Wee1 association with Hsp90 and Wee1 stability.
  • Yeast expressing non-phosphorylatable Hsp90 (yHsp90-Y24F) exhibit premature nuclear division.
  • These cells display G2/M checkpoint defects, similar to swe1∆ yeast, indicating impaired cell cycle regulation.

Conclusions:

  • Hsp90 phosphorylation by Wee1 is a key regulatory mechanism for cell cycle control.
  • This phosphorylation event is crucial for maintaining proper cell division and checkpoint function.
  • Targeting Hsp90 phosphorylation may offer novel therapeutic strategies in cancer treatment.