Time-lapse imaging of embryonic neural stem cell division in Drosophila by two-photon microscopy

This unit describes a protocol for live imaging of Drosophila embryonic neural stem cells using two-photon microscopy. Compared to traditional one-photon confocal imaging, this technique renders higher-resolution optical sections from deeper within the embryo. It is ideally suited to following embryonic neuroblasts located underneath the neuroepithelial cell layer for several rounds of cell division.