Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of Platelet-Bound C4d (PC4d) in Predicting Risk of Future Thrombotic Events in Systemic Lupus Erythematosus.

Arthritis care & research·2023

Same author

Antiphospholipid antibodies are persistently positive at high titers. Additive value of platelet-bound C4d.

Frontiers in immunology·2022

Same author

Capillary Blood Levels of Hydroxychloroquine and Methotrexate Are Stable for up to 5 Years When Collected on Volumetric Absorptive Microsamplers.

The journal of applied laboratory medicine·2021

Same author

A Bayesian framework for efficient and accurate variant prediction.

PloS one·2018

Same author

Pharmacogenetic profile and major depressive and/or bipolar disorder treatment: a retrospective, cross-sectional study.

Pharmacogenomics·2018

Same author

TumorNext-Lynch-MMR: a comprehensive next generation sequencing assay for the detection of germline and somatic mutations in genes associated with mismatch repair deficiency and Lynch syndrome.

Oncotarget·2018

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

Published on: January 4, 2015

High resolution array-CGH characterization of human stem cells using a stem cell focused microarray.

Aaron M Elliott¹, Kristi A Hohenstein Elliott, Anja Kammesheidt

¹Ambry Genetics, Aliso Viejo, CA 92656, USA. aelliott@ambrygen.com

Molecular Biotechnology

|June 5, 2010

Summary

This summary is machine-generated.

Genomic instability in human stem cells (ESCs, iPSCs) can compromise research and therapies. A new high-resolution microarray detects small genomic aberrations, improving stem cell characterization and experimental reliability.

More Related Videos

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Related Experiment Videos

Last Updated: Jun 12, 2026

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

Published on: January 4, 2015

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Area of Science:

Stem cell biology
Genomics
Molecular genetics

Background:

Human embryonic and induced pluripotent stem cells (ESCs, iPSCs) are prone to genomic instability during extended culture.
Chromosomal aberrations in stem cells reduce experimental reproducibility and therapeutic potential.
Current karyotype analysis offers low-resolution detection of large aneuploidies, necessitating improved methods.

Purpose of the Study:

To develop and validate a high-resolution, cost-effective microarray for detecting genomic aberrations in human stem cells.
To enhance the characterization of stem cell lines and ensure their suitability for research and therapy.

Main Methods:

Design of a custom array-comparative genomic hybridization (aCGH) microarray with high probe density for stem cell and cancer-related genes.
Analysis of induced pluripotent stem cell (iPSC) lines using the custom microarray.
Comparison of results with standard karyotyping and a conventional 44K microarray.

Main Results:

The custom microarray identified genomic abnormalities missed by karyotyping and standard microarrays.
Several small duplications affecting stem cell and/or cancer-related genes were detected.
The focused microarray provides higher resolution detection of structural variants.

Conclusions:

The stem cell-focused aCGH microarray is a reliable, high-resolution tool for detecting genomic aberrations in human ESCs and iPSCs.
This technology improves the characterization of stem cell lines, increasing confidence in experimental results and therapeutic applications.