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Published on: January 22, 2018
Downregulation of SPARC expression decreases gastric cancer cellular invasion and survival
Jie Yin1, Guowei Chen, Yucun Liu
1Department of General Surgery, Peking University First Hospital, Beijing, 100034, PR China.
Background:
Secreted protein acidic and rich in cysteine (SPARC) plays a key role in the development of many tissues and organ types. Aberrant SPARC expression was found in a wide variety of human cancers, contributes to tumor development. Because SPARC was found to be overexpressed in human gastric cancer tissue, we therefore to explore the expression of SPARC in gastric cancer lines and the carcinogenic mechanisms.
Methods:
SPARC expression was evaluated in a panel of human gastric cancer cell lines. MGC803 and HGC 27 gastric cancer cell lines expressing high level of SPARC were transiently transfected with SPARC-specific small interfering RNAs and subsequently evaluated for effects on invasion and proliferation.
Results:
Small interfering RNA-mediated knockdown of SPARC in MGC803 and HGC 27 gastric cancer cells dramatically decreased their invasion. Knockdown of SPARC was also observed to significantly increase the apoptosis of MGC803 and HGC 27 gastric cancer cells compared with control transfected group.
Conclusions:
Our data showed that downregulating of SPARC inhibits invasion and growth of human gastric cancer cells. Thus, targeting of SPARC could be an effective therapeutic approach against gastric cancer.
Insights
Downregulating secreted protein acidic and rich in cysteine (SPARC) inhibits gastric cancer cell invasion and growth. Targeting SPARC may offer a new therapeutic strategy for treating gastric cancer.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Secreted protein acidic and rich in cysteine (SPARC) is crucial for tissue development.
- Aberrant SPARC expression is implicated in various human cancers.
- SPARC is overexpressed in gastric cancer tissues.
Purpose of the Study:
- To investigate SPARC expression in gastric cancer cell lines.
- To explore the role of SPARC in gastric cancer progression and identify potential therapeutic targets.
Main Methods:
- SPARC expression analysis in human gastric cancer cell lines.
- RNA interference (RNAi) using SPARC-specific small interfering RNAs (siRNAs) in MGC803 and HGC 27 cells.
- Assessment of cell invasion, proliferation, and apoptosis following SPARC knockdown.
Main Results:
- SPARC knockdown significantly reduced invasion in MGC803 and HGC 27 gastric cancer cells.
- Reduced SPARC levels led to increased apoptosis in these cell lines.
- SPARC inhibition demonstrated a direct impact on gastric cancer cell behavior.
Conclusions:
- Downregulation of SPARC effectively inhibits the invasion and growth of human gastric cancer cells.
- Targeting SPARC presents a promising therapeutic strategy for gastric cancer treatment.
- Further research into SPARC-targeted therapies is warranted.
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