Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

Published on: August 6, 2020

Hypothesis testing for neural cell growth experiments using a hybrid branching process model.

Kingshuk Roy Choudhury¹, Pearl Deacon, Rob Barrett

¹Statistics Department, University College Cork, Cork, Ireland. kingshuk@ucc.ie

Biostatistics (Oxford, England)

|June 8, 2010

Summary

We developed a new model to analyze neuron branching patterns, offering insights into neural development and disease. This method accurately quantifies branching using counts, not complex topology, enabling broader studies of neural networks.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of Colorized Display of Mammograms on Lesion Detection.

Journal of breast imaging·2024

Same author

Impact of the COVID-19 pandemic on prescription refills for immune-mediated inflammatory disorders: a time series analysis (January 2019 to January 2021) using the English Prescribing Dataset.

BMJ open·2022

Same author

Long-term evolution of LI-RADS observations in HCV-related cirrhosis treated with direct-acting antivirals.

Liver international : official journal of the International Association for the Study of the Liver·2021

Same author

Corrigendum to "Impact of baseline covariate imbalance on bias in treatment effect estimation in cluster randomized trials: Race as an example" [Contemporary Clinical Trials volume 88 (2020) 105775].

Contemporary clinical trials·2021

Same author

Patient-Informed Organ Dose Estimation in Clinical CT: Implementation and Effective Dose Assessment in 1048 Clinical Patients.

AJR. American journal of roentgenology·2021

Same author

Early <sup>18</sup>F-FDG-PET Response During Radiation Therapy for HPV-Related Oropharyngeal Cancer May Predict Disease Recurrence.

International journal of radiation oncology, biology, physics·2020

Area of Science:

Neuroscience
Computational Biology
Biostatistics

Background:

Neuron branching patterns are crucial for understanding neural cell types, development, and neurodegenerative diseases.
Existing models often require detailed branching topology, limiting sample size and applicability.

Purpose of the Study:

To develop a novel hybrid Markovian model for analyzing neural branching patterns.
To enable analysis using only branch counts, thus allowing for larger sample sizes.
To investigate the effects of growth factor (GF), hypothalamic-astroglial conditioned medium (HY), and combination treatments on neuron branching.

Main Methods:

A hybrid Markovian model incorporating a discretized gamma distribution for primary branching underdispersion.
Inclusion of both bifurcation and trifurcation events.

More Related Videos

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

Published on: August 23, 2016

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

Published on: July 24, 2019

Related Experiment Videos

Last Updated: Jun 12, 2026

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

Published on: August 6, 2020

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

Published on: August 23, 2016

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

Published on: July 24, 2019

Estimation of probabilities using a gamma generalized linear model and an expectation-maximization algorithm for incomplete data.

Development of a unified likelihood ratio test (LRT) methodology for hypothesis testing.

Main Results:

The model demonstrated statistically adequate fit across all branching orders.
GF, HY, and combination treatments significantly increased primary and secondary branching compared to control (p < 0.01).
Bifurcation probabilities decreased exponentially with branching order for GF and combination treatments, but not HY (p=0.03).

Conclusions:

The developed model provides a robust and flexible framework for analyzing neuron branching patterns using accessible data.
Specific treatments influence early-stage neuron branching, suggesting targeted genetic regulation.
The findings offer new avenues for studying neural development and disease mechanisms.