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Molecular characterization and epidemiology of rotavirus isolates obtained from children with diarrhoea in Malaysia

H Zuridah1, C D Kirkwood, R F Bishop

  • 1Faculty of Health Sciences, Universiti Teknologi MARA, Jalan Othman, 46000 Petaling Jaya, Selangor, Malaysia. drzuridah@salam.uitm.edu.my

The Medical Journal of Malaysia
|June 10, 2010
PubMed

Insights

Rotavirus G/P types shifted significantly between 1996 and 2007, with G9 becoming prevalent. This genetic evolution in human rotaviruses highlights the need for updated rotavirus vaccines.

Area of Science:

  • Virology
  • Molecular Epidemiology
  • Public Health

Background:

  • Rotavirus is a leading cause of severe diarrheal disease in infants and young children globally.
  • Understanding the genetic diversity and evolution of rotavirus strains is crucial for vaccine development and public health strategies.

Purpose of the Study:

  • To investigate the changes in rotavirus G/P genotypes between 1996 and 2007.
  • To analyze the genetic diversity of human group A rotaviruses over an 11-year period.

Main Methods:

  • Retrospective analysis of rotavirus RNA samples from 1996 and 2007.
  • Genotyping using hemi-nested PCR for G and P types.
  • Phylogenetic analysis to study genetic diversity.

Main Results:

  • In 1996, G1P[8] was dominant (81%).
  • By 2007, G9 (42%) emerged as a prevalent genotype, alongside G1P[8] (33%) and G1P[UT] (25%).
  • Electropherotype analysis showed mostly long patterns with minor variations; phylogenetic analysis revealed genetic diversity.

Conclusions:

  • Significant shifts in rotavirus G/P genotype prevalence occurred between 1996 and 2007.
  • The emergence of G9 genotype necessitates consideration for rotavirus vaccine efficacy.
  • Findings support informed decisions regarding rotavirus vaccine introduction in the coming decade.

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