Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Primitive Basic Amino Acids Promote Mineral-Catalyzed Electrochemical Reduction of H<sup>+</sup> and CO<sub>2</sub>.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same author

Preserved Antegrade Pulmonary Blood Flow in Bidirectional Glenn: Outcomes and Considerations for Staged Palliation.

The Annals of thoracic surgery·2026
Same author

Associating the phenotypic expression of platelets with disease type through image-based single-cell profiling.

Thrombosis research·2026
Same author

<sup>16</sup>O poor cosmic spherules from near-Earth CY chondrite asteroids.

Science advances·2026
Same author

Lansoprazole-Induced Microscopic Colitis Mimicking Anticancer Drug-Related Diarrhea During Lenvatinib and Pembrolizumab Combination Therapy: A Case Report.

Clinical case reports·2026
Same author

When wet meets dry: An Ivuna-like impactor triggered volatile loss on the angrite parent body.

Science advances·2026

Related Experiment Video

Updated: Jun 12, 2026

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination
04:53

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

Published on: April 5, 2022

A novel method for isolating podocytes using magnetic activated cell sorting.

Ayumi Murakami1, Hisashi Oshiro, Seiichi Kanzaki

  • 1Department of Pathology, Yokohama City University School of Medicine 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|June 10, 2010
PubMed
Summary

Researchers developed a new method to quickly isolate mouse podocytes using magnetic activated cell sorting. This technique aids in studying podocyte biology and proteinuria mechanisms.

More Related Videos

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
10:20

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

Published on: August 10, 2018

Immunomagnetic Isolation of the Vascular Wall-Resident CD34+ Stem Cells from Mice
08:14

Immunomagnetic Isolation of the Vascular Wall-Resident CD34+ Stem Cells from Mice

Published on: December 22, 2023

Related Experiment Videos

Last Updated: Jun 12, 2026

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination
04:53

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

Published on: April 5, 2022

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
10:20

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

Published on: August 10, 2018

Immunomagnetic Isolation of the Vascular Wall-Resident CD34+ Stem Cells from Mice
08:14

Immunomagnetic Isolation of the Vascular Wall-Resident CD34+ Stem Cells from Mice

Published on: December 22, 2023

Area of Science:

  • Nephrology
  • Cell Biology
  • Immunology

Background:

  • Podocytes are crucial in preventing proteinuria.
  • Mechanisms of podocyte injury remain unclear due to isolation challenges.
  • A novel method for rapid podocyte isolation is needed.

Purpose of the Study:

  • To develop a rapid and efficient method for isolating mouse podocytes.
  • To enable further research into podocyte biology and injury mechanisms.

Main Methods:

  • Endothelial cells were depleted using anti-CD31 antibody.
  • Nephrin-positive cells were isolated using magnetic activated cell sorting (MACS) with anti-nephrin antibody.
  • Cell purity and marker expression were analyzed via confocal microscopy, FACS, PCR, and immunoblotting.

Main Results:

  • Isolated cells showed dual positivity for synaptopodin and rabbit IgG.
  • MACS yielded approximately 75% pure podocyte fractions within 6 hours.
  • Sorted cells exhibited significantly higher expression of podocyte-specific molecules.

Conclusions:

  • The novel MACS method provides a rapid way to isolate mouse podocytes.
  • This technique is valuable for genomic, proteomic, and transcriptomic analyses.
  • It facilitates in vivo molecular profiling of podocyte biology without tissue culture.