Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Local Anesthetic Bupivacaine Inhibits the Progression of Non-Small Cell Lung Cancer by Inducing Autophagy Through Akt/mTOR Signaling.

Frontiers in oncology·2021

Same author

Light exposure mediates circadian rhythms of rhizosphere microbial communities.

The ISME journal·2021

Same author

Novel Potent Selective Orally Active S1P5 Receptor Antagonists.

ACS medicinal chemistry letters·2021

Same author

Biodegradable metals for bone fracture repair in animal models: a systematic review.

Regenerative biomaterials·2021

Same author

Rectangular multilayer dielectric gratings with broadband high diffraction efficiency and enhanced laser damage resistance.

Optics express·2021

Same author

Invariant Image Representation Using Novel Fractional-Order Polar Harmonic Fourier Moments.

Sensors (Basel, Switzerland)·2021

Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026

Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026

Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026

Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026

Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026

Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

ZOOM Lite: next-generation sequencing data mapping and visualization software.

Zefeng Zhang¹, Hao Lin, Bin Ma

¹Bioinformatics Solutions Inc., Waterloo, ON N2L 6J2, Canada.

Nucleic Acids Research

|June 10, 2010

Summary

This summary is machine-generated.

ZOOM Lite is new software for efficient next-generation sequencing data analysis. It accurately maps millions of reads and visualizes results, simplifying bioinformatics pipelines for researchers.

More Related Videos

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

Related Experiment Videos

Last Updated: Jun 12, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

Area of Science:

Bioinformatics and Computational Biology
Genomics and Genetics
Software Development for Life Sciences

Background:

High-throughput sequencing generates massive datasets requiring efficient analysis tools.
Existing software often lacks integration of mapping and visualization, or user-friendly interfaces.
Demands for speed, accuracy, and usability in sequencing data analysis are increasing.

Purpose of the Study:

To introduce ZOOM Lite, a novel software solution for next-generation sequencing data analysis.
To provide an efficient and accurate platform for reads mapping and result visualization.
To integrate these processes into an easy-to-use pipeline for desktop computers.

Main Methods:

Development of a high-performance kernel for mapping millions of Illumina or AB SOLiD sequencing reads.
Implementation of an intuitive graphical user interface (GUI) for seamless workflow.
Support for both single-end and paired-end reads, with flexible output options (unique or top N mappings).

Main Results:

ZOOM Lite demonstrates efficient and accurate mapping of tens of millions of sequencing reads.
The software integrates reads mapping and visualization into a user-friendly desktop pipeline.
It accommodates various input formats and outputs to standard result formats.

Conclusions:

ZOOM Lite offers a valuable tool for researchers needing efficient and integrated next-generation sequencing data analysis.
The software enhances usability and accessibility of complex bioinformatics tasks.
Its availability facilitates broader adoption and application in genomic research.