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Updated: Jun 12, 2026

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
Published on: July 14, 2023
Experimental varicocele induces p53-dependent germ cell apoptosis through activation of γ-H2AX
In-Youb Chang1, Jin-Ho Kim, Kyeong-Han Park
1Department of Anatomy, College of Medicine, Chosun University, Gwangju, Republic of Korea.
Aim:
Since DNA damage-related apoptosis is raising concerns regarding abnormal spermatogenesis, we investigated the changes in γ-H2AX during testicular germ cell apoptotic responses in the varicocele model.
Materials And Methods:
Varicocele was induced by partial ligation of the left renal vein and animals were sacrificed at 1, 3, and 4 weeks after varicocele creation. The levels of activated p53 and γ-H2AX formation were determined by Western blot analysis and immunohistochemistry.
Results:
γ-H2AX formation was augmented after varicocele creation, while a significant increase in p53 phosphorylation was detected in a time course-dependent manner. Varicocele-dependent nuclear γ-H2AX staining in the primary spermatocytes was prominent as degenerative foci, while little differences could be detected in spermatogonia.
Conclusions:
These results show that experimental varicocele may induce p53-dependent apoptosis through activation of γ-H2AX in the primary spermatocytes, and suggest that γ-H2AX may be related to apoptotic signal transduction in experimental varicocele.
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