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Related Experiment Video

Updated: Jun 12, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Quantitative cell-based reporter gene assays using droplet-based microfluidics.

Jean-Christophe Baret1, Yannick Beck, Isabelle Billas-Massobrio

  • 1Institut de Science et d'Ingénierie Supramoléculaires, Université de Strasbourg, CNRS UMR 7006, 8 Allée Gaspard Monge, BP 70028, F-67083 Strasbourg Cedex, France. jc.baret@unistra.fr

Chemistry & Biology
|June 11, 2010
PubMed
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This summary is machine-generated.

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This study introduces a droplet microfluidic system for precise single-cell analysis of nuclear receptor ligands. The method accurately quantifies hormone dose-response profiles, enabling robust EC50 measurements.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Nuclear receptors play crucial roles in cellular processes.
  • Accurate quantification of nuclear receptor ligand activity is essential for research and drug discovery.
  • Existing methods for ligand screening can be time-consuming and require large sample volumes.

Purpose of the Study:

  • To develop a droplet-based microfluidic system for quantitative, single-cell reporter gene assays.
  • To enable precise measurement of nuclear receptor ligand dose-response profiles at the single-cell level.
  • To validate the system's accuracy and efficiency using 20-hydroxyecdysone in Bombyx mori cells.

Main Methods:

  • Utilized a droplet-based microfluidic system to encapsulate single Bombyx mori cells in nanoliter droplets.

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Last Updated: Jun 12, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
07:37

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Published on: June 26, 2016

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12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

  • Generated discrete concentrations of 20-hydroxyecdysone on-chip via serial dilution, encoded by fluorescent labels.
  • Simultaneously measured green fluorescent protein (GFP) reporter gene expression and fluorescent label concentration for dose-response analysis.

    • Main Results:

    • Screened approximately 7500 cells per concentration, yielding statistically relevant data.
    • Successfully constructed single-cell dose-response profiles for 20-hydroxyecdysone.
    • Precisely measured the EC50 as 70 nM +/- 12% (alpha = 0.05), consistent with literature values.

    Conclusions:

    • Droplet microfluidics offers a highly sensitive and efficient platform for cell-based reporter gene assays.
    • The developed system allows for precise, single-cell level quantification of nuclear receptor ligand activity.
    • This approach provides a valuable tool for high-throughput screening and detailed mechanistic studies of nuclear receptor signaling.