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2D and 3D Matrices to Study Linear Invadosome Formation and Activity
Published on: June 2, 2017
The structure of invadopodia in a complex 3D environment
Ondrej Tolde1, Daniel Rösel, Pavel Veselý
1Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
European Journal of Cell Biology
|June 12, 2010
Summary
Invadopodia morphology differs in 3D environments. In a dense matrix, invadopodia have a thick base and thin filaments, with matrix degradation occurring at the base, not the protrusions.
Area of Science:
- Cell biology
- Biochemistry
- Extracellular matrix research
Background:
- Invadopodia and podosomes are crucial for extracellular matrix degradation.
- Previous studies primarily used thin matrices, potentially misrepresenting in vivo structures.
Purpose of the Study:
- To investigate invadopodial morphology in a complex 3D environment.
- To compare invadopodial structures on dense, dermis-based matrices versus thin substrates.
Main Methods:
- Culturing cells on a cell-free dermis-based matrix.
- Observing invadopodial formation and morphology using microscopy.
- Analyzing the distribution of F-actin, phospho-paxillin, phospho-cortactin, and phosphotyrosine.
Main Results:
- Invadopodia exhibit distinct morphology on dense matrices compared to thin substrates.
- On dermis-based matrices, invadopodia feature a thick base with F-actin and signaling proteins, and numerous thin, F-actin-rich filaments.
- Matrix-degrading activity is localized to the invadopodial base.
Conclusions:
- Invadopodial structure and function are influenced by the 3D matrix environment.
- This study provides a more physiologically relevant model for invadopodia research.
- Findings can inform new criteria for identifying invadopodia in vivo and studying related signaling pathways.
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