Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Positron Emission Tomography

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body being...

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Imaging Studies III: Computed Tomography

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Microbial characterization of oral microbiome in patients with open-angle glaucoma.

Scientific reports·2026

Same author

ASO Visual Abstract: Comparing Resident Team Performance in Complex Surgical Oncology: A Single-Institution Cohort Study.

Annals of surgical oncology·2026

Same author

Patient Outcome Reports for Senior Residents: An Opportunity to Foster Reflection and Identify Outliers for Intervention.

Journal of surgical education·2026

Same author

Emergency hernia hospitalizations in older adults with and without multimorbidity.

Hernia : the journal of hernias and abdominal wall surgery·2026

Same author

An Assessment of Hospital Performance in Emergency General Surgery Using Direct and Indirect Standardization.

Annals of surgery open : perspectives of surgical history, education, and clinical approaches·2026

Same author

A Multi-State Cohort Study of Hospital Utilization and Treatment Modality for Malignant Gastrointestinal Obstructions.

Journal of surgical oncology·2026

Same journal

TRABD2A promotes osteogenic differentiation of human periodontal ligament stem cells by modulating TNF-α and IL-1β.

Organogenesis·2026

Same journal

Thyroid cancer-derived exosomal SPP1 promotes tumor progression by driving macrophage M2 polarization through the CD44/JAK2/STAT3 signaling pathway.

Organogenesis·2026

Same journal

MLPH/RAB3A accelerates the differentiation of pancreatic stem cells to islet β-cells to control blood glucose in diabetic rats.

Organogenesis·2026

Same journal

Adipose-derived mesenchymal stem cells-derived exosomes containing nano-pearl powder water-soluble matrix promote osteogenic differentiation of MC3T3-E1 cells.

Organogenesis·2026

Same journal

Comparison of vascular remodeling between a bioresorbable poly-L-lactic acid scaffold and a bare metal stent: a 6-month angiography and intravascular ultrasound analysis in porcine iliac arteries.

Organogenesis·2026

Same journal

Optimization of TX-100/SDS-based decellularized vascular material using ultrasound and chemical treatment: evaluation of structure and biosafety.

Organogenesis·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Live optical projection tomography.

Jean-François Colas¹, James Sharpe

¹EMBL-CRG Systems Biology Program; Centre for Genomic Regulation; UPF; Barcelona, Spain; Istituciô Catalana de Recerca i Estudis Avançats; Barcelona, Spain.

|June 12, 2010

Summary

This summary is machine-generated.

Optical projection tomography (OPT) advances live imaging of embryonic organs. This 4D OPT technology provides quantitative developmental data for computational modeling and new biological insights.

Keywords:

blood circulation conceptus culture ex utero ex vivo heartbeat limb bud live imaging milieu intérieur optical tomography organogenesis

More Related Videos

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography

Published on: July 29, 2011

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

Related Experiment Videos

Last Updated: Jun 12, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography

Published on: July 29, 2011

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

Area of Science:

Developmental biology
Biomedical imaging
Molecular imaging

Background:

Optical projection tomography (OPT) is effective for imaging embryonic organs.
Live imaging is crucial for understanding developmental dynamics.

Purpose of the Study:

Highlight successes in live OPT for organogenesis research.
Discuss advancements in live OPT by integrating mouse embryo culture.
Explore future applications of this molecular imaging technique.

Main Methods:

Utilizing optical projection tomography (OPT) for live imaging.
Implementing time-lapse OPT (4D OPT) for developmental studies.
Integrating state-of-the-art mouse embryo culture with OPT.

Main Results:

Live OPT enables quantitative data accumulation on organogenesis.
Successful integration of mouse embryo culture with OPT system.
Demonstrated potential for broader applications of live OPT.

Conclusions:

Live OPT is a powerful tool for studying organogenesis dynamics.
Technological integration enhances live OPT capabilities for embryo imaging.
Further development promises expanded applications in molecular imaging.