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Related Experiment Video

Updated: Jun 12, 2026

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
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Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Published on: September 8, 2023

Asymmetric PPCys: strongly fluorescing NIR labels.

Georg Michael Fischer1, Christian Jüngst, Magnus Isomäki-Krondahl

  • 1Fachbereich Chemie and Center of Applied Photonics, Universität Konstanz, 78457 Konstanz, Germany.

Chemical Communications (Cambridge, England)
|June 12, 2010
PubMed
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Researchers developed novel Pyrrolopyrrole Cyanines (PPCy) with unique asymmetric structures. These PPCy compounds show potent near-infrared absorption and fluorescence, with demonstrated cellular uptake via peptide targeting.

Area of Science:

  • Organic chemistry
  • Photophysics
  • Bioconjugation

Background:

  • Pyrrolopyrrole Cyanines (PPCy) are a class of dyes with potential applications in imaging and sensing.
  • Developing PPCy with tailored optical properties and targeted delivery remains a challenge.

Purpose of the Study:

  • To synthesize biofunctionalized Pyrrolopyrrole Cyanines (PPCy) with asymmetric substitution.
  • To investigate their photophysical properties, specifically near-infrared (NIR) absorption and fluorescence.
  • To demonstrate the cellular internalization of a peptide-conjugated PPCy.

Main Methods:

  • Stepwise synthesis strategy for creating asymmetric PPCy.
  • Spectroscopic analysis to determine NIR absorption and fluorescence characteristics.
  • Live cell microscopy to track the internalization of peptide-bound PPCy.

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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Published on: December 11, 2021

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Last Updated: Jun 12, 2026

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
05:54

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Published on: September 8, 2023

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Main Results:

  • Successfully obtained biofunctionalized PPCy with an asymmetric substitution pattern.
  • PPCy exhibited exceptionally strong and narrowband NIR absorption and fluorescence.
  • Demonstrated successful internalization of a peptide-bound PPCy into live cells.

Conclusions:

  • The developed asymmetric PPCy possess superior NIR optical properties.
  • Peptide conjugation enables targeted cellular uptake of PPCy.
  • These findings pave the way for advanced bioimaging applications using novel PPCy.