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Updated: Jun 12, 2026
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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
Published on: March 25, 2015
Structural polymorphism of non-covalent peptide-based delivery systems: highway to cellular uptake
Sébastien Deshayes1, Karidia Konate, Gudrun Aldrian
1University of Montpellier, Department of Molecular Biophysics and Therapeutics, 34293 Montpellier, France.
Biochimica Et Biophysica Acta
|June 15, 2010
Summary
Cell-penetrating peptides (CPPs) form nanoparticles for effective therapeutic delivery. This review explores CPP nanoparticle mechanisms, formation, and cellular uptake for in vivo applications.
Area of Science:
- Biotechnology
- Nanomedicine
- Drug Delivery
Background:
- Therapeutic molecule delivery faces significant challenges in clinical development.
- Viral and non-viral strategies exist, but few reach preclinical evaluation.
- Cell-penetrating peptides (CPPs) offer a promising strategy for in vivo therapeutic delivery.
Purpose of the Study:
- To review the mechanisms of peptide-based nanoparticles (MPG, PEP, CADY).
- To examine the structural and biophysical context of these nanoparticles.
- To highlight factors influencing particle formation, stabilization, and cellular uptake.
Main Methods:
- Focus on structural and biophysical analysis of MPG, PEP, and CADY.
- Review of parameters affecting nanoparticle formation and stability.
- Analysis of how carrier structural polymorphism impacts cellular uptake.
Main Results:
- Peptide-based nanoparticles efficiently deliver therapeutic cargoes.
- MPG, PEP, and CADY systems demonstrate successful ex vivo and in vivo delivery.
- Cellular uptake is influenced by nanoparticle structure and formation parameters.
Conclusions:
- Peptide-based nanoparticles are a viable strategy for systemic in vivo delivery.
- Understanding structural and biophysical properties is key to optimizing CPP delivery systems.
- Further research into carrier polymorphism can enhance therapeutic targeting and efficacy.
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